Journal: Journal of Ginseng Research

Article Title: The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

doi: 10.1016/j.jgr.2023.07.003

Figure Lengend Snippet: EPG increased viability, and reduced injury biomarkers and [Ca2+]i in hypoxia cardiomyocytes via p-caveolin-1 (three independent experiments done in quintuplicate). Effect of hypoxia time (A) and EPG doses under hypoxia for 6 h (B) on cells. Effect of PP2 on cells (C) and LDH, BNP and cTnI (D-F) under 200 μg/mL EPG. Fluo-3 fluorescence measured by microscope (G) and flow cytometry (H, I). ###P < 0.001, ##P < 0.01 vs. normal control; ∗∗∗P < 0.001, ∗∗P < 0.01vs. model, & P < 0.05, && P < 0.01, &&& P < 0.001 vs. EPG.

Article Snippet: The culture medium and the serum were collected and analyzed by lactate dehydrogenase (LDH) kit (Jiancheng Bio, Nanjing, China), B-type natriuretic peptide (BNP) ELISA kit (Zci Bio, Shanghai, China) and cardiac Troponin I (cTnI) ELISA kit (Novatein Bio, USA) according to the instructions.

Techniques: Fluorescence, Microscopy, Flow Cytometry, Control

Journal: Journal of Ginseng Research

Article Title: The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

doi: 10.1016/j.jgr.2023.07.003

Figure Lengend Snippet: EPG improved myocardial infarct and injury biomarkers in rats with MI via p-caveolin-1 (n = 6-10/group). (A) TTC staining. (B) Infarction size (%). (C-E) Release of LDH, BNP and cTnI (C-E). ###P < 0.001 vs. sham; ∗∗P < 0.01, ∗∗∗P < 0.001, vs. model; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. EPG.

Article Snippet: The culture medium and the serum were collected and analyzed by lactate dehydrogenase (LDH) kit (Jiancheng Bio, Nanjing, China), B-type natriuretic peptide (BNP) ELISA kit (Zci Bio, Shanghai, China) and cardiac Troponin I (cTnI) ELISA kit (Novatein Bio, USA) according to the instructions.

Techniques: Staining

Journal: Experimental Animals

Article Title: Downregulation of interferon-induced protein with tetratricopeptide repeats 3 relieves the inflammatory response and myocardial fibrosis of mice with myocardial infarction and improves their cardiac function

doi: 10.1538/expanim.21-0060

Figure Lengend Snippet: Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) knockdown alleviated cardiac injury and the inflammatory response 3 days after myocardial infarction (MI). (A, B) The levels of cTnI and CK-MB in serum were detected using commercial ELISA kits. (C) Representative images of myocardial tissues stained with HE (magnification ×200, scale bar=100 µ m). (D) Representative fluorescent immunostaining images of CD68 + macrophages and quantification of CD68-positive cells (magnification ×400, scale bar=50 µ m). (E) Relative mRNA levels of inflammatory cytokines were determined using quantitative real-time PCR (qRT-PCR). β-actin served as the internal control. The data are expressed as the mean ± SD (n=6 per group). ** P <0.01; *** P <0.001.

Article Snippet: The serum levels of cardiac troponin-I (cTnI) and creatine kinase-MB (CK-MB) were measured using a mouse cTnI ELISA kit (EM1466, FineTest, Wuhan, China) and mouse CK-MB ELISA kit (EM0929, FineTest) according to the manufacturer’s instructions.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Staining, Immunostaining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control